non-muscle heavy chain 10 Myosin Antibody (GT246) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13591

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT246

Format

Azide and BSA Free
View all non-muscle heavy chain 10 Myosin Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human non-muscle heavy chain 10 Myosin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

229 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:5000.
Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunohistochemistry-Paraffin: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
non-muscle heavy chain 10 Myosin Antibody (GT246) - Azide and BSA Free

Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] -

MYH10 antibody [GT246] detects MYH10 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MYH10 stained by MYH10 antibody [GT246] (NBP3-13591) diluted at 1:250.
Blue: Fluoroshield with DAPI.

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
non-muscle heavy chain 10 Myosin Antibody (GT246) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] -

MYH10 antibody [GT246] detects MYH10 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MYH10 protein stained by MYH10 antibody [GT246] (NBP3-13591) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: non-muscle heavy chain 10 Myosin

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Long Name

Myosin-10

Alternate Names

MYH10, NMMHC II-b, NMMHC-B, NMMHC-IIB

Gene Symbol

MYH10

Additional non-muscle heavy chain 10 Myosin Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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