non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13532
![Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]](https://resources.rndsystems.com/images/products/non-muscle-Myosin-IIA-Antibody-GT218-Western-Blot-NBP3-13532-img0003.jpg "Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]")
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Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (99%), Canine (99%), Chicken (95%), Rat (97%), Xenopus (91%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # GT218
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
Centrifuge briefly prior to opening.
Scientific Data Images for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free
Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]
Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]](https://resources.rndsystems.com/images/products/non-muscle-Myosin-IIA-Antibody-GT218-Immunohistochemistry-Paraffin-NBP3-13532-img0002.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]")
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532]
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.
non-muscle Myosin IIA stained by non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.
non-muscle Myosin IIA stained by non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -
Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - non-muscle Myosin IIA antibody [GT218] detects non-muscle Myosin IIA protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: non-muscle Myosin IIA stained by non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -
Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT218] (NBP3-13532) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] -
Western Blot: non-muscle Myosin IIA Antibody (GT218) [NBP3-13532] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [GT218] diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Non-muscle Myosin IIA
Long Name
Myosin-9
Alternate Names
MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA
Gene Symbol
MYH9
Additional Non-muscle Myosin IIA Products
Product Documents for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for non-muscle Myosin IIA Antibody (GT218) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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