non-muscle Myosin IIA [p Ser1943] Antibody

Name: non-muscle Myosin IIA [p Ser1943] Antibody
Brand: Novus Biologicals
SKU: NBP3-23432
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23432

Submit a review

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all Non-muscle Myosin IIA Primary Antibodies »

Product Specifications

Immunogen

Phospho-non-muscle Myosin IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse non-muscle Myosin IIA, but is not found in non-muscle Myosin IIB and is not well conserved in non-muscle Myosin IIC. [UniProt# P35579]

Modification

p Ser1943

Specificity

This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for non-muscle Myosin IIA [p Ser1943] Antibody

Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody [NBP3-23432] -

Western Blot: non-muscle Myosin IIA [p Ser1943] Antibody [NBP3-23432] - Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

Applications for non-muscle Myosin IIA [p Ser1943] Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Non-muscle Myosin IIA

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Long Name

Myosin-9

Alternate Names

MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA

Gene Symbol

MYH9

Additional Non-muscle Myosin IIA Products

Product Documents for non-muscle Myosin IIA [p Ser1943] Antibody

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for non-muscle Myosin IIA [p Ser1943] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for non-muscle Myosin IIA [p Ser1943] Antibody

There are currently no reviews for this product. Be the first to review non-muscle Myosin IIA [p Ser1943] Antibody and earn rewards!

Have you used non-muscle Myosin IIA [p Ser1943] Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image