NOSIP Antibody - BSA Free

Immunoprecipitation:NOSIP AntibodyNBP3-29170] -

Immunoprecipitation:NOSIP AntibodyNBP3-29170] -Detection of human NOSIP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOSIP antibody NBP3-29170 (lot NBP3-29170-1) used for IP at 6 µg per reaction. NOSIP was also immunoprecipitated by rabbit anti-NOSIP antibody. For blotting immunoprecipitated NOSIP, NBP3-29170 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot :NOSIP AntibodyNBP3-29170] -

Western Blot :NOSIP AntibodyNBP3-29170] -Whole cell lysate (50 µg) from HeLa, HEK293T,Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells preparedusing NETN lysis buffer. Antibody: Affinity purified rabbitanti-NOSIP antibody usedfor WB at 0.1 µg/ml. Detection: Chemiluminescence withan exposure time of 30 seconds.