NOV/CCN3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88155

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NOV/CCN3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:LPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGVEVSDSSVNCIEQTTEWTACSKSCGMGFSTRVTNRNRQCEMLKQTRL

Reactivity Notes

Possible cross-reactivity based on sequence identity: Mouse (81%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOV/CCN3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155]

Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155]

Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155] - CCN3 Antibody [NBP1-88155] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.
NOV/CCN3 Antibody - BSA Free Western Blot: NOV/CCN3 Antibody - BSA Free [NBP1-88155]

Western Blot: NOV/CCN3 Antibody - BSA Free [NBP1-88155]

Analysis in control (vector only transfected HEK293T lysate) and NOV over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for NOV/CCN3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOV/CCN3

CCN3 is encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Long Name

Nephroblastoma Overexpressed Gene

Alternate Names

CCN3, IGFBP-9

Entrez Gene IDs

4856 (Human)

Gene Symbol

CCN3

UniProt

P48745

Additional NOV/CCN3 Products

Product Documents for NOV/CCN3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NOV/CCN3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for NOV/CCN3 Antibody - BSA Free

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  • Q: I am looking for the expression of CCN3/NOV in tumor cells and I am interested in finding an antibody that has a lot of data to support the antibody is working well. Can you make a recommendation?

    A:

    We have two antibodies, NBP1-88154 and NBP1-88155 that have been extremely well characterized by testing at the Human Protein Atlas. Please see the Human Protein Atlas website for full testing for these products.

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail