NOXO1 Antibody

Novus Biologicals | Catalog # NB300-950

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NOXO1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence RGCVDSVPHPTTEQ corresponding to C-Terminus according to NP_653204.1, NP_751907.1, NP_751907.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28824611).

Specificity

This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NOXO1 Antibody

Immunohistochemistry-Paraffin: NOXO1 Antibody [NB300-950]

Immunohistochemistry-Paraffin: NOXO1 Antibody [NB300-950]

Immunohistochemistry-Paraffin: NOXO1 Antibody [NB300-950] - IHC analysis of NOXO1 in formalin-fixed, paraffin-embedded human kidneyusing this antibody at 10 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NOXO1 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 20 ug/ml

Immunohistochemistry-Paraffin

1:10 - 1:500

Peptide ELISA

Detection limit 1:16000

Western Blot

1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave no signal but low background in Hela and human heart lysates at up to 1 ug/ml. IHC-P: Human kidney cortex shows cytoplasmic stain in epithelial cells of some PCT.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NOXO1

NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 co-localizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.

Alternate Names

MGC20258, NADPH oxidase organizer 1, Nox organizer 1, Nox-organizing protein 1, P41NOX, P41NOXA, P41NOXB, P41NOXC, regulatory protein P41NOX, SH3 and PX domain-containing protein 5, SH3PXD5NADPH oxidase regulatory protein, SNX28

Entrez Gene IDs

124056 (Human)

Gene Symbol

NOXO1

UniProt

NP_653204 (Human)

Additional NOXO1 Products

Product Documents for NOXO1 Antibody

Certificate of Analysis

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Product Specific Notices for NOXO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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