NPRA/NPR1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31333

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human, Mouse

Predicted:

Bovine (97%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NPRA/NPR1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NPRA/NPR1. The exact sequence is proprietary.

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NPRA/NPR1 Antibody - BSA Free

Western Blot: NPRA/NPR1 Antibody [NBP1-31333]

Western Blot: NPRA/NPR1 Antibody [NBP1-31333]

Western Blot: NPRA/NPR1 Antibody [NBP1-31333] - HL-60 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NPR-A antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
NPRA/NPR1 Antibody - BSA Free

Western Blot: NPRA/NPR1 Antibody - BSA Free [NBP1-31333] -

Insulin signaling, de novo lipogenesis, adipogenesis, lipolysis, and PKG activity in adipose tissues of HFD-fed NprcAKO mice.(A to C) Western blotting analysis for proteins involved in insulin signaling (p-Ser473 AKT, p-Thr308 AKT, and GLUT4), de novo lipogenesis (ACC and FASN), adipogenesis (PPAR gamma, C/EBP alpha, and ADIPOQ), PKG activity (p-Ser239 VASP), and lipolysis (p-Ser563 HSL and ATGL) performed on lysates from gWAT (A), iWAT (B), and BAT (C) of NprcAKO and Nprcfl/fl mice after 12 weeks on HFD. p, phosphorylated. Blots are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28743802), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NPRA/NPR1 Antibody - BSA Free

Western Blot: NPRA/NPR1 Antibody - BSA Free [NBP1-31333] -

Insulin signaling, de novo lipogenesis, adipogenesis, lipolysis, and PKG activity in adipose tissues of HFD-fed NprcAKO mice.(A to C) Western blotting analysis for proteins involved in insulin signaling (p-Ser473 AKT, p-Thr308 AKT, and GLUT4), de novo lipogenesis (ACC and FASN), adipogenesis (PPAR gamma, C/EBP alpha, and ADIPOQ), PKG activity (p-Ser239 VASP), and lipolysis (p-Ser563 HSL and ATGL) performed on lysates from gWAT (A), iWAT (B), and BAT (C) of NprcAKO and Nprcfl/fl mice after 12 weeks on HFD. p, phosphorylated. Blots are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28743802), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
NPRA/NPR1 Antibody - BSA Free

Western Blot: NPRA/NPR1 Antibody - BSA Free [NBP1-31333] -

Insulin signaling, de novo lipogenesis, adipogenesis, lipolysis, and PKG activity in adipose tissues of HFD-fed NprcAKO mice.(A to C) Western blotting analysis for proteins involved in insulin signaling (p-Ser473 AKT, p-Thr308 AKT, and GLUT4), de novo lipogenesis (ACC and FASN), adipogenesis (PPAR gamma, C/EBP alpha, and ADIPOQ), PKG activity (p-Ser239 VASP), and lipolysis (p-Ser563 HSL and ATGL) performed on lysates from gWAT (A), iWAT (B), and BAT (C) of NprcAKO and Nprcfl/fl mice after 12 weeks on HFD. p, phosphorylated. Blots are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28743802), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NPRA/NPR1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NPRA/NPR1

Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 (PubMed 7982997)). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).(supplied by OMIM)

Long Name

Natriuretic Peptide Receptor A

Alternate Names

ANPa, ANPRA, GC-A, GUCY2A, NPR1, NPRA, Pndr

Gene Symbol

NPR1

Additional NPRA/NPR1 Products

Product Documents for NPRA/NPR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NPRA/NPR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NPRA/NPR1 Antibody - BSA Free

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