Mouse Nrf2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-76758

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.188ng/mL

Assay Range

0.313 - 20ng/mL
View all Nrf2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.13

Inter-Assay Precision (Precision between assays) CV% 5.39

Recovery for Mouse Nrf2 ELISA Kit (Colorimetric)

Recovery

86-107%

Linearity

Mouse Nrf2 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Nrf2 ELISA Kit (Colorimetric)

Mouse Nrf2 ELISA Kit (Colorimetric)

ELISA: Mouse Nrf2 ELISA Kit (Colorimetric) [NBP2-76758] -

ELISA: Mouse Nrf2 ELISA Kit (Colorimetric) [NBP2-76758] - Standard Curve Reference

Kit Contents for Mouse Nrf2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol

NFE2L2

Additional Nrf2 Products

Product Documents for Mouse Nrf2 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse Nrf2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse Nrf2 ELISA Kit (Colorimetric)

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Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail