Nucleolin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-1920

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Nucleolin Primary Antibodies »

Product Specifications

Immunogen

A 15 residue peptide designed to the C-terminus of human Nucleolin [UniProt# P19338]

Localization

Nucleoli

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Nucleolin Antibody - Azide and BSA Free (NB100-1920) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Nucleolin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nucleolin Antibody - Azide and BSA Free

Western Blot: Nucleolin Antibody [NB100-1920]

Western Blot: Nucleolin Antibody [NB100-1920]

Nucleolin-Antibody-Western-Blot-NB100-1920-img0010.jpg
Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB100-1920]

Immunocytochemistry/ Immunofluorescence: Nucleolin Antibody [NB100-1920]

Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [NB100-1920] - Detection of Nucleolin (green) in Hela cells detected with a Dylight-488 conjugated secondary antibody. Nuclei are counter stained with Hoechst 33258.
Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920]

Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920]

Immunohistochemistry-Paraffin: Nucleolin Antibody [NB100-1920] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:750 conc. of Nucleolin antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed in normal cells and nuclear staining was observed in cancer cells. Staining was performed by Histowiz.
Western Blot: Nucleolin Antibody [NB100-1920]

Western Blot: Nucleolin Antibody [NB100-1920]

Western Blot: Nucleolin Antibody [NB100-1920] - Analysis of Nucleolin in HeLa nuclear extracts.

Applications for Nucleolin Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:3000-1:10000

Immunohistochemistry

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Immunoprecipitation

1-3ug

Western Blot

1:1000 - 1:2000
Application Notes
This Nucleolin antibody is useful in Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nucleolin

Nucleolin (NCL, also called Protein C23) is a major phosphoprotein present in nucleolus of growing eukaryotic cells which is implicated in the synthesis as well as maturation of ribosomes. Localized mainly to nucleolus, NCL may also be found in cytoplasmic mRNP granules containing untranslated mRNAs (mRNP granule complex contains - NCL, ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs). NCL interacts with APTX, NSUN2, TERT, ERBB4, GZF1, GZF1, NVL and C1QBP, and is a part of SWAP complex (NPM1, NCL, PARP1 and SWAP70) as well as another complex containing NCL, HTATSF1/Tat-SF1, CDK9 and CCNT1, RNA polymerase II, SUPT5H. NCL may be found associated with intranucleolar chromatin and pre-ribosomal particles where it induces chromatin de-condensation via binding to histone H1 and play a key role in pre-rRNA transcription as well as ribosome assembly. NCL has also been suggested to implicate in the process of transcriptional elongation and it binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Besides being essential to rRNA processing in nucleolus, NCL has several other functions such as transcription regulation, modulation of mRNA stability and DNA replication and recombination.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Entrez Gene IDs

4691 (Human); 25135 (Rat)

Gene Symbol

NCL

UniProt

P19338 (Human)

Additional Nucleolin Products

Product Documents for Nucleolin Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Nucleolin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nucleolin Antibody - Azide and BSA Free

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Protocols

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