NuMA Antibody (1C5) - Azide and BSA Free

Novus Biologicals | Catalog # H00004926-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C5

Format

Azide and BSA Free
View all NuMA Primary Antibodies »

Product Specifications

Immunogen

NUMA1 (NP_006176, 200 a.a. ~ 306 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEK

Localization

Nuclear

Specificity

NUMA1 - nuclear mitotic apparatus protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NuMA Antibody (1C5) - Azide and BSA Free

Western Blot: NuMA Antibody (1C5) [H00004926-M01]

Western Blot: NuMA Antibody (1C5) [H00004926-M01]

Western Blot: NuMA Antibody (1C5) [H00004926-M01] - NUMA1 monoclonal antibody (M01), clone 1C5 Analysis of NUMA1 expression in K-562.
Immunocytochemistry/ Immunofluorescence: NuMA Antibody (1C5) [H00004926-M01]

Immunocytochemistry/ Immunofluorescence: NuMA Antibody (1C5) [H00004926-M01]

Immunocytochemistry/Immunofluorescence: NuMA Antibody (1C5) [H00004926-M01] - Immunofluorescence of monoclonal antibody to NUMA1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunoprecipitation: NuMA Antibody (1C5) [H00004926-M01]

Immunoprecipitation: NuMA Antibody (1C5) [H00004926-M01]

Immunoprecipitation: NuMA Antibody (1C5) [H00004926-M01] - Analysis of NUMA1 transfected lysate using anti-NUMA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NUMA1 MaxPab rabbit polyclonal antibody.
ELISA: NuMA Antibody (1C5) [H00004926-M01]

ELISA: NuMA Antibody (1C5) [H00004926-M01]

ELISA: NuMA Antibody (1C5) [H00004926-M01] - Detection limit for recombinant GST tagged NUMA1 is approximately 0.1ng/ml as a capture antibody.

Applications for NuMA Antibody (1C5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NuMA

NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis.

Long Name

Nuclear Mitotic Apparatus Protein 1

Alternate Names

NMP-22, NUMA1, SP-H Antigen

Entrez Gene IDs

4926 (Human)

Gene Symbol

NUMA1

OMIM

164009 (Human)

UniProt

NP_006176

Additional NuMA Products

Product Documents for NuMA Antibody (1C5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NuMA Antibody (1C5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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