O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

Novus Biologicals | Catalog # NBP2-75601

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JB44-39 expressed in HEK293
View all O-GlcNAc Transferase/OGT Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human O-GlcNAc Transferase p110 subunit aa 997-1,046 / 1,046. (SwissProt: O15294 Human; SwissProt: Q8CGY8 Mouse; SwissProt: P56558 Rat)

Localization

Nucleus. Cytoplasm. Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Western Blot: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of OGT on Hela (1) and SiHa (2) cell lysate using anti-OGT antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Staining OGT in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded rat epididymis tissue using anti-OGT antibody. Counter stained with hematoxylin.
Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Flow Cytometry: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of Hela cells with OGT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Staining OGT in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunocytochemistry/ Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunocytochemistry/Immunofluorescence: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Staining OGT in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded human liver tissue using anti-OGT antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded human kidney tissue using anti-OGT antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601]

Immunohistochemistry-Paraffin: O-GlcNAc Transferase p110 subunit Antibody (JB44-39) [NBP2-75601] - Analysis of paraffin-embedded mouse testis tissue using anti-OGT antibody. Counter stained with hematoxylin.

Applications for O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: O-GlcNAc Transferase/OGT

Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.

Long Name

O-Linked N-Acetylglucosamine (GlcNAc) Transferase

Alternate Names

HRNT1, OGlcNAc Transferase, OGT

Gene Symbol

OGT

Additional O-GlcNAc Transferase/OGT Products

Product Documents for O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

Certificate of Analysis

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Product Specific Notices for O-GlcNAc Transferase p110 subunit Antibody (JB44-39)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

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