ODF2 Antibody (JE35-01)

Novus Biologicals | Catalog # NBP3-32676

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE35-01 expressed in HEK293
View all ODF2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human ODF2 aa 780-829 / 829. (Uniprot: Q5BJF6)

Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cell projection, cilium, microtubule organizing center, centriole, spindle pole, flagellum.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ODF2 Antibody (JE35-01)

ODF2 Antibody (JE35-01)

Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676]

Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676] - Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (NBP3-32676) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: K-562 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: U-2 OS cell lysate
Lane 6: Jurkat cell lysate
Lane 7: HepG2 cell lysate

Lysates/proteins at 15 ug/Lane.

Predicted band size: 95 kDa
Observed band size: 95 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32676) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for ODF2 Antibody (JE35-01)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ODF2

Cenexin-1, also known as ODF2 and outer dense fiber of sperm tails 2, are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene; however, not all isoforms and variants have been fully described.

Alternate Names

Cenexin, FLJ44866, MGC111096, MGC9034, ODF2/1, ODF2/2, ODF8484-kD, outer dense fiber of sperm tails 2, outer dense fiber protein 2, sperm tail structural protein

Gene Symbol

ODF2

Additional ODF2 Products

Product Documents for ODF2 Antibody (JE35-01)

Certificate of Analysis

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Product Specific Notices for ODF2 Antibody (JE35-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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