OG 488 BAPTA-1 AM

Tocris Bioscience | Catalog # 6256

Cell-permeable, fluorescent Ca2+ indicator
Tocris Bioscience

Key Product Details

Description

Cell-permeable, fluorescent Ca2+ indicator

Wavelength

Excitation = 494 nm, Emission = 523 nm
View all Calcium Signaling Probes and Ca2+ Indicator Dyes »

Product Description

OG 488 BAPTA-1 AM is a cell-permeable, fluorescently labeled Ca2+ indicator (Kd =170 nM). Composed of a green fluorescent probe (OG 488, Cat. No. 6257) conjugated to a cell-permeable Ca2+ chelator (BAPTA AM, Cat. No. 2787). Fluorescent intensity increases approximately 14-fold from resting intensity, upon Ca2+ binding. Displays uniform cellular distribution with minimal subcellular compartmentalization. Excitation/emission λ 494/523 nm.

Optical Data

Emission Color Green
λabs 494 nm
λem 523 nm
Extinction Coefficient (ε) 19000 M-1cm-1
Quantum Yield (φ) 0.91
Closest Laser line 488 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescence Microscopy

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Product Specifications for OG 488 BAPTA-1 AM

Molecular Weight

1258.06

Formula

C59H53F2N3O26

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[4-[[[3',6'-bis(acetyloxy)-2',7'-difluoro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]amino]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]phenoxy]ethoxy]phenyl]glycine (acetyloxy)methyl ester

CAS Number

244167-57-5

PubChem ID

102028682

InChI Key

NQVWLDWXFNDFLA-UHFFFAOYSA-N

SMILES

FC1=CC2=C(C3=CC=C(C(NC4=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC5=CC=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4)=O)C=C3C([O-])=O)C6=CC(F)=C(OC(C)=O)C=C6[O+]=C2C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 1.26 1

Preparing Stock Solutions for OG 488 BAPTA-1 AM

The following data is based on the product molecular weight 1258.06.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.01 mM 79.49 mL 397.44 mL 794.87 mL
0.05 mM 15.90 mL 79.49 mL 158.97 mL
0.1 mM 7.95 mL 39.74 mL 79.49 mL
0.5 mM 1.59 mL 7.95 mL 15.90 mL

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Background References

References are publications that support the biological activity of the product.

Product Documents for OG 488 BAPTA-1 AM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for OG 488 BAPTA-1 AM

For research use only

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