OLFM-L3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15055

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (96%), Rat (93%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all OLFM-L3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human OLFM-L3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for OLFM-L3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: OLFM-L3 Antibody [NBP2-15055]

Immunocytochemistry/ Immunofluorescence: OLFM-L3 Antibody [NBP2-15055]

Immunocytochemistry/Immunofluorescence: OLFM-L3 Antibody [NBP2-15055] - Analysis of methanol-fixed A549, using Olfactomedin-like 3 antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: OLFM-L3 Antibody [NBP2-15055]

Immunohistochemistry-Paraffin: OLFM-L3 Antibody [NBP2-15055]

Immunohistochemistry-Paraffin: OLFM-L3 Antibody [NBP2-15055] - Mouse heart. Olfactomedin-like 3 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
OLFM-L3 Antibody

Western Blot: OLFM-L3 Antibody [NBP2-15055] -

Western Blot: OLFM-L3 Antibody [NBP2-15055] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OLFML3 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.

Applications for OLFM-L3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: OLFM-L3

Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.

Long Name

Olfactomedin-Like 3

Alternate Names

HNOEL-iso, OLF44, OLFML3

Gene Symbol

OLFML3

UniProt

Q9NRN5 (Human)

Additional OLFM-L3 Products

Product Documents for OLFM-L3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for OLFM-L3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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