Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-29523

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Guinea Pig (100%), Human (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Olig2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 conjugated to KLH. Accession # Q13516

Modification

p Ser14, p Ser10, p Ser13

Marker

Oligodendrocyte Marker

Specificity

Specific for endogenous levels of the ~32 kDa Olig2 phosphorylated at Ser10, Ser 13, and Ser14. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

Western Blot: Olig2 [p Ser14, p Ser10, p Ser13] Antibody [NBP2-29523]

Western Blot: Olig2 [p Ser14, p Ser10, p Ser13] Antibody [NBP2-29523]

Western Blot: OLIG2 [p Ser14, p Ser10, p Ser13] Antibody [NBP2-29523] - Western blot of rat neonatal brain lysate showing specific immunolabeling of the ~ 32k form of the Olig2 protein phosphorylated at Ser10,13,14. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).

Applications for Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Olig2

Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).

Long Name

Oligodendrocyte Lineage Transcription Factor 2

Alternate Names

BHLHB1, PRKCBP2, RACK17

Entrez Gene IDs

10215 (Human)

Gene Symbol

OLIG2

UniProt

Q13516

Additional Olig2 Products

Product Documents for Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Olig2 [p Ser14, p Ser10, p Ser13] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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