Opsin 3 Antibody - BSA Free

Novus Biologicals | Catalog # NLS2134

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Monkey (94%), Primate (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Opsin 3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from C-terminus of human Opsin 3.

Epitope

C-Terminus

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21700660). Predicted cross-reactivity based on sequence identity: Gibbon (94%), Marmoset (94%). Rat reactivity reported from a verified customer review.

Localization

Cell Membrane

Specificity

Human Opsin 3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for Opsin 3 Antibody - BSA Free

Immunohistochemistry Free-Floating: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry Free-Floating: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry Free-Floating: Opsin 3 Antibody [NLS2134] - Rat brain samples (cerebral cortex). Rat brain 40um free floating sections were incubated with Opsin 3 Antibody (NLS2134) at 1:250 overnight at 4 degrees C. The following day, sections were incubated with anti-rabbit Alexa Fluor Plus 488 1:500 and DAPI. Opsin 3 Antibody (NLS2134; 1:250) in green. Nuclei stained with DAPI (blue). IHC image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Analysis of anti-Encephalopsin / OPN3 antibody with brain, neurons and glia at 5 ug/mL.
Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Analysis of anti-Encephalopsin / OPN3 antibody with human skin, melanoma.
Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody - BSA Free [NLS2134]

Immunohistochemistry-Paraffin: Opsin 3 Antibody [NLS2134] - Anti-OPN3 / Encephalopsin antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for Opsin 3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 - 8 ug/mL
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID: 21700660). This Opsin 3 Antibody is validated for IHC-FrFl from a verified customer review..

Reviewed Applications

Read 1 review rated 3 using NLS2134 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: Opsin 3

Encephalopsin, a member of the Opsin subfamily of GPCRs, is similar in sequence to vertebrate retinal and pineal opsins and may affect photoreception in the brain. Encephalopsin expression has been documented in various brain tissues, including the preoptic area and paraventricular nucleus of the hypothalamus, cerebral cortex, cerebellar Purkinje neurons, a subset of striatal neurons, selected thalamic nuclei, and a subset of interneurons in the ventral horn of the spinal cord. ESTs have been isolated from human blood, breast, cervix, colon, germ cell, head/neck, kidney, lung, pancreas, placenta, prostate, skin, testis, and uterus cancer libraries, as well as from normal human blood, brain, eye, heart, kidney, liver, liver/spleen, ovary, placenta, prostate, skin, testis, and B-cell/lung/testis libraries.

Alternate Names

ECPN, ECPNopsin-3, Encephalopsin, ERO, NMO-1, opsin 3, opsin 3 (encephalopsin, panopsin), Panopsin

Entrez Gene IDs

23596 (Human)

Gene Symbol

OPN3

Additional Opsin 3 Products

Product Documents for Opsin 3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Opsin 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Opsin 3 Antibody - BSA Free

Customer Reviews for Opsin 3 Antibody - BSA Free (1)

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Customer Images

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  • Opsin 3 Antibody
    Name: Soledad Barez
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain free floating slices
    Species: Rat
    Verified Customer | Posted 10/26/2021
    Rat brain samples (cerebral cortex). Opsin 3 Antibody (NLS2134; 1:250) in green. Nuclei stained with DAPI (blue)
    Rat brain 40um free floating sections were incubated with Opsin 3 Antibody (NLS2134) at 1:250 overnight at 4°C. The following day, sections were incubated with anti-rabbit Alexa Fluor Plus 488 1:500 and DAPI.
    Opsin 3 Antibody - BSA Free NLS2134
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

View specific protocols for Opsin 3 Antibody - BSA Free (NLS2134):

Immunohistochemistry Protocol for Opsin 3 Antibody (NLS2134):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene approximately 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol approximately 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol approximately 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol approximately 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water approximately 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block approximately 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody approximately 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T approximately 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary approximately 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
17. Apply an alkaline phosphatase steptavidin approximately 30 minutes at RT.
18. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
19. Apply an alkaline phosphatase chromagen substrate approximately 30 minutes at RT.
20. Rinse the slide in distilled water approximately 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol approximately 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol approximately 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol approximately 1 minute each @ RT.
24. Wash the slides in 3 changes of xyleneapproximately 1 minute each @ RT.
25. Apply cover slip.

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