Orai1 is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. Orai1 is a pore subunit of Ca(2+) release-activated Ca(2+) CRAC channels, and STIM1 acts as an endoplasmic reticulum (ER) Ca(2+) sensor that detects store depletion. This type of channel is the primary way for calcium influx into T-cells. Defects in Orai1 are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1.
Orai1 Antibody (anti-ORAI1) [DyLight 680]
Novus Biologicals | Catalog # NBP3-27962FR
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional
Label
DyLight 680 (Excitation = 692 nm, Emission = 712 nm)
Antibody Source
Recombinant Monoclonal Human IgG2 Clone # anti-ORAI1 expressed in CHO
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Product Specifications
Immunogen
Orai1
Clonality
Monoclonal
Host
Human
Isotype
IgG2
Description
The heavy chain type is huIgG2, and the light chain type is hulambda. It has a predicted MW of 145.5 kDa.
Applications for Orai1 Antibody (anti-ORAI1) [DyLight 680]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Orai1
Long Name
ORAI Calcium Release-activated Calcium Modulator 1
Alternate Names
CRACM1, ORAT1, TMEM142A
Gene Symbol
ORAI1
Additional Orai1 Products
Product Documents for Orai1 Antibody (anti-ORAI1) [DyLight 680]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Orai1 Antibody (anti-ORAI1) [DyLight 680]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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