Rat ORP150/HSP12A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-76587

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.188ng/mL

Assay Range

0.313 - 20ng/mL
View all ORP150/HSP12A ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat HYOU1 in samples. No significant cross-reactivity or interference between Rat HYOU1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.05

Inter-Assay Precision (Precision between assays) CV% 5.33

Recovery for Rat ORP150/HSP12A ELISA Kit (Colorimetric)

Recovery

88-109%

Linearity

Rat ORP150/HSP12A ELISA Kit (Colorimetric)

Scientific Data Images for Rat ORP150/HSP12A ELISA Kit (Colorimetric)

Rat ORP150/HSP12A ELISA Kit (Colorimetric)

ELISA: Rat ORP150/HSP12A ELISA Kit (Colorimetric) [NBP2-76587] -

ELISA: Rat ORP150/HSP12A ELISA Kit (Colorimetric) [NBP2-76587] - Standard Curve Reference

Kit Contents for Rat ORP150/HSP12A ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ORP150/HSP12A

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Long Name

Oxygen Regulated Protein, 150 kDa

Alternate Names

HSP12A, HYOU1

Gene Symbol

HYOU1

Additional ORP150/HSP12A Products

Product Documents for Rat ORP150/HSP12A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat ORP150/HSP12A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

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