Oxytocin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-80261

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

9.375 pg/mL

Assay Range

15.625-1000 pg/mL
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Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

This kit recognizes oxytocin in samples. No significant cross-reactivity or interference between oxytocin and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.35%

Inter-Assay Precision (Precision between assays) CV% 4.67%

Recovery for Oxytocin ELISA Kit (Colorimetric)

Recovery

88-104%

Linearity

Oxytocin ELISA Kit (Colorimetric)

Scientific Data Images for Oxytocin ELISA Kit (Colorimetric)

Oxytocin ELISA Kit (Colorimetric)

ELISA: Oxytocin ELISA Kit (Colorimetric) [NBP2-80261] -

ELISA: Oxytocin ELISA Kit (Colorimetric) [NBP2-80261] - Standard Curve Reference

Kit Contents for Oxytocin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Oxytocin

The nonapeptide hormones Arginine Vasopressin (ARG-Vasopressin or AVP) and Oxytocin are synthesized in the supraoptic and paraventricular nuclei of the hypothalamus together with their respective "carrier" proteins, the neurophysins. Vasopressin and Oxytocin are produced by separate populations of magnocellular neurons in both nuclei. Neurophysin I (NPI) and Neurophysin II (NPII) function as carrier proteins for Oxytocin and Vasopressin, respectively. Oxytocin is a pituitary hormone which induces uterine contractions during childbirth and the ejection of milk from the mammary glands during nursing. Vasopressin is involved in the metabolism of water and electrolytes and has been identified as a vasoconstrictor. Both Neurophysin genes exist as three exons, with each exon encoding a functional protein domain. Studies show that the identically conserved middle region (exon B) is involved in NP-NP homodimer formation as well as being the site for the Glycine 17 to Valine point mutation responsible for familial diabetes insipidus. The genes encoding Neurophysin I and II map to human chromosome 20p13.

Alternate Names

Neurophysin I, OT, OXT

Gene Symbol

OXT

Additional Oxytocin Products

Product Documents for Oxytocin ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Oxytocin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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