Catalog Number: 4485
Chemical Name: 1-[5-[(2,4-Difluorophenyl)thio]-4-nitro-2-thienyl]-ethanone
Biological Activity
Inhibitor of ubiquitin-specific protease (USP) 7 (EC50 = 8.6 μM); also inhibits the closely related deubiquitinase (DUB) USP47. Demonstrates downstream inhibition of HDM2 and claspin in vitro. Inhibits USP7-mediated p53 deubiquitination. Blocks deubiquitination of Tip60 (KAT5) histone lysine acetyltransferase.
Technical Data
  • M.Wt:
    315.32
  • Formula:
    C12H7F2NO3S2
  • Solubility:
    Soluble to 100 mM in DMSO
  • Purity:
    >99%
  • Storage:
    Store at +4°C
  • CAS No:
    1247819-59-5
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Deubiquitylase, deSUMOylase, and deISGylase activity microarrays for assay of substrate preference and functional modifiers.
    Loch et al.
    Mol.Cell.Proteomics, 2011;10:M100.002402
  2. Characterization of selective ubiquitin and ubiquitin-like protease inhibitors using a fluorescence-based multiplex assay format.
    Tian et al.
    Assay Drug Dev.Technol., 2011;9:165
  3. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes.
    Altun et al.
    Chem.Biol., 2011;18:1401
  4. Deubiquitination of Tip60 by USP7 determines the activity of the p53-dependent apoptotic pathway.
    Dar et al.
    Mol.Cell Biol., 2013;33:3309
Citations:

The citations listed below are publications that use Tocris products. Selected citations for P 22077 include:

1 Citations: Showing 1 - 1

  1. USP7 cooperates with SCML2 to regulate the activity of PRC1.
    Authors: Lecona Et al.
    Pharmacol Res Perspect 2015;35:1157

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