P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

Novus Biologicals | Catalog # NB100-65392

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Equine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Psel.KO.2.7

Format

BSA Free
View all P-Selectin/CD62P Primary Antibodies »

Product Specifications

Immunogen

P-selectin transfected 300.19 cells

Reactivity Notes

Predicted cross-reactivities: Mouse, Equine, Goat, Bovine, Feline, Sheep, Rat

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

Immunocytochemistry/ Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Immunocytochemistry/ Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Immunocytochemistry/Immunofluorescence: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Alignment of live-cell calcium response behavior with PTH abundance in a parathyroid adenoma. (A) Prestimulation baseline flux activity at time 0; (B) same field of view at 2 minutes after stimulation with 2 mM calcium; (C) same field after fixation and immunofluorescent staining for PTH (red); (D) image overlay of (B) and (C). Flux images (A, B) are single timepoint frames from the same time-ordered image stack. Anti-PTH reactivity is visualized by an AlexaFluor555 secondary antibody. Magnification = 200X. Image using the Allophycocyanin format of this antibody.
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) labeled with anti-CD62P (Psel.KO.2.7) compared to equine platelets labeled with murine IgG-DyLight 488 isotype control (gray fill).
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets.
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Staining of thrombin activated human platelets
Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free [NB100-65392]

Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [NB100-65392] - Analysis using the DyLight 488 conjugate of NB100-65392. Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.

Applications for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. IHC-Frozen use reported in literature (see Massaguer, A. et al, 2000). Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 31237986).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: P-Selectin/CD62P

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Entrez Gene IDs

6403 (Human)

Gene Symbol

SELP

UniProt

P16109

Additional P-Selectin/CD62P Products

Product Documents for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does this antibody come conjugated to anything other than PE?

    A: NB100-65392, clone Psel.KO.2.7 is only available conjugated to PE. We do sell kits that allow you to conjugate it to another color of your choice if you are interested. We can also do the conjugation in house for an additional fee and this can usually be done in 5-7 business days, depending on how busy the lab is. PE is our only readily available conjugation without an additional fee though.

  • Q: I am considering purchasing NB100-65392. Do you have a recommended isotype control to use in conjunction?

    A: NBP1-43778 is an appropriate isotype control.

  • Q: The datasheet states that it is an IgG1 antibody. Is this IgG1 kappa or IgG1 lambda?

    A: It is IgG1 kappa.

  • Q: Does this antibody come conjugated to anything other than PE?

    A: NB100-65392, clone Psel.KO.2.7 is only available conjugated to PE. We do sell kits that allow you to conjugate it to another color of your choice if you are interested. We can also do the conjugation in house for an additional fee and this can usually be done in 5-7 business days, depending on how busy the lab is. PE is our only readily available conjugation without an additional fee though.

  • Q: I am considering purchasing NB100-65392. Do you have a recommended isotype control to use in conjunction?

    A: NBP1-43778 is an appropriate isotype control.

  • Q: The datasheet states that it is an IgG1 antibody. Is this IgG1 kappa or IgG1 lambda?

    A: It is IgG1 kappa.

  • Q: Does this antibody come conjugated to anything other than PE?

    A: NB100-65392, clone Psel.KO.2.7 is only available conjugated to PE. We do sell kits that allow you to conjugate it to another color of your choice if you are interested. We can also do the conjugation in house for an additional fee and this can usually be done in 5-7 business days, depending on how busy the lab is. PE is our only readily available conjugation without an additional fee though.

  • Q: I am considering purchasing NB100-65392. Do you have a recommended isotype control to use in conjunction?

    A: NBP1-43778 is an appropriate isotype control.

  • Q: The datasheet states that it is an IgG1 antibody. Is this IgG1 kappa or IgG1 lambda?

    A: It is IgG1 kappa.

Showing  1 - 3 of 3 FAQs Showing All
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