p114RhoGEF Antibody (1972) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43546

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1972

Format

Azide and BSA Free
View all p114RhoGEF Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human p114RhoGEF. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p114RhoGEF Antibody (1972) - Azide and BSA Free

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:1000.
Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:3000
Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546]

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:1000
Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546]

Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546]

Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546] - Rat lymph node. ARHGEF18 stained by ARHGEF18 antibody [1972] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p114RhoGEF Antibody (1972) - Azide and BSA Free

Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546] -

ARHGEF18 antibody [GT1972] detects ARHGEF18 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
ARHGEF18 stained by ARHGEF18 antibody [GT1972] (NBP2-43546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p114RhoGEF Antibody (1972) - Azide and BSA Free

Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARHGEF18 antibody [GT1972] (NBP2-43546) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for p114RhoGEF Antibody (1972) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 30948425).

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: p114RhoGEF

Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Alternate Names

114 kDa Rho-specific guanine nucleotide exchange factor, EC 3.4.24, EC 3.6.1, KIAA0521rho guanine nucleotide exchange factor 18, MGC15913, p114RhoGEF, P114-RhoGEF, P114-RHO-GEF, Rho/Rac guanine nucleotide exchange factor (GEF) 18, Rho/Rac guanine nucleotide exchange factor 18, Rho-specific guanine nucleotide exchange factor p114, SA-RhoGEF, Septin-associated RhoGEF

Gene Symbol

ARHGEF18

Additional p114RhoGEF Products

Product Documents for p114RhoGEF Antibody (1972) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p114RhoGEF Antibody (1972) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for p114RhoGEF Antibody (1972) - Azide and BSA Free

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Protocols

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FAQs

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