p130Cas/BCAR1 Antibody (JE36-56)

Novus Biologicals | Catalog # NBP3-32071

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE36-56 expressed in HEK293
View all p130Cas Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human p130Cas/BCAR1 aa 1-100 / 870. (Uniprot: P56945)

Localization

Cell junction, focal adhesion, Cytoplasm, Cell projection, axon.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p130Cas/BCAR1 Antibody (JE36-56)

p130Cas/BCAR1 Antibody (JE36-56)

Western Blot: p130Cas/BCAR1 Antibody (JE36-56) [NBP3-32071]

Western Blot: p130Cas/BCAR1 Antibody (JE36-56) [NBP3-32071] - Western blot analysis of p130Cas/BCAR1 on different lysates with Rabbit anti-p130Cas/BCAR1 antibody (NBP3-32071) at 1/1,000 dilution.

Lane 1: HUVEC cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-2 OS cell lysate
Lane 4: HEK-293 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 93 kDa
Observed band size: 110 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32071) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for p130Cas/BCAR1 Antibody (JE36-56)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p130Cas

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 Antibody (JE36-56)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for p130Cas/BCAR1 Antibody (JE36-56)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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