p130Cas/BCAR1 [p Tyr165] Antibody

Novus Biologicals | Catalog # NBP3-13075

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all p130Cas Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr165 of human p130Cas/BCAR1. The exact sequence is proprietary.

Modification

p Tyr 165

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for p130Cas/BCAR1 [p Tyr165] Antibody

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)
Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075]

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas/BCAR1 (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
p130Cas/BCAR1 [p Tyr165] Antibody

Immunocytochemistry/ Immunofluorescence: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] -

p130Cas (phospho Tyr165) antibody detects p130Cas (phospho Tyr165) protein at cell junction by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: p130Cas (phospho Tyr165) protein stained by p130Cas (phospho Tyr165) antibody (NBP3-13075) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
p130Cas/BCAR1 [p Tyr165] Antibody

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (NBP3-13075) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
p130Cas/BCAR1 [p Tyr165] Antibody

Western Blot: p130Cas/BCAR1 [p Tyr165] Antibody [NBP3-13075] -

Untreated (-) and treated (+) NIH-3T3 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p130Cas (phospho Tyr165) antibody (NBP3-13075) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for p130Cas/BCAR1 [p Tyr165] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p130Cas

BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells. Alternate names for BCAR1/p130CAS include breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, CAS scaffolding protein family member 1, CAS, and CRKAS.

Long Name

Breast Cancer Anti-estrogen Resistance 1

Alternate Names

BCAR1, CASS1, CRKAS

Gene Symbol

BCAR1

Additional p130Cas Products

Product Documents for p130Cas/BCAR1 [p Tyr165] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for p130Cas/BCAR1 [p Tyr165] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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