p73 Antibody (5B1288) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33241

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B1288

Format

Azide and BSA Free
View all p73 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).

Specificity

It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. NB100-56674 has been shown to recongize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). The antibody does not cross react with p53.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p73 Antibody (5B1288) - Azide and BSA Free

Western Blot: p73 Antibody (5B1288)Azide Free [NBP2-33241]

Western Blot: p73 Antibody (5B1288)Azide Free [NBP2-33241]

Western Blot: p73 Antibody (5B1288) - Azide Free [NBP2-33241] - Analysis of p73 in transfected cell lysate using this antibody. Image from the standard format of this antibody.
Immunocytochemistry: p73 Antibody (5B1288) - Azide Free [NBP2-33241]

Immunocytochemistry: p73 Antibody (5B1288) - Azide Free [NBP2-33241]

Immunocytochemistry: p73 Antibody (5B1288) - Azide Free [NBP2-33241] - Human breast cancer section using 10 ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxida
Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - Azide Free [NBP2-33241]

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - Azide Free [NBP2-33241]

Immunohistochemistry-Paraffin: p73 Antibody (5B1288) - Azide Free [NBP2-33241] - Human breast cancer section using 10ug/mL of p73 antibody (clone 5B1288) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidas
Western Blot: p73 Antibody (5B1288)Azide Free [NBP2-33241]

Western Blot: p73 Antibody (5B1288)Azide Free [NBP2-33241]

Western Blot: p73 Antibody (5B1288) - Azide Free [NBP2-33241] - Analysis of p73 in HeLa cell lysate (Cat no. NBP2-25045) using this antibody at 1 ug/mL. Image from the standard format of this antibody.

Applications for p73 Antibody (5B1288) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500. Use reported in scientific literature (Accardi et al)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (Sayan et al)

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (Sayan et al)

Western Blot

1-3 ug/ml
Application Notes
Immunoprecipitation details can be found in Sayan et al (2005). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: p73

p73 is a transcription factor that shares a significant amino acid identity with p53 and p63 (1). p73 and p63 can both activate p53-responsive genes and induce apoptosis upon over expression (2) Unlike p53, p73 is not induced by DNA damage and is not a target for inactivation by viral oncoproteins (3). Similar to p63, p73 has an additional coding region in the C-terminus (not found in p53) that can undergo complex alternative splicing giving rise to 3 splice forms (alpha, beta, and gamma) (4). Activation of p73 requires the presence of functional c-Abl. It has also be found that mutant p53 can bind to and inactivate p73 (5).

Long Name

Tumor Protein p73

Alternate Names

TP73

Gene Symbol

TP73

Additional p73 Products

Product Documents for p73 Antibody (5B1288) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for p73 Antibody (5B1288) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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