PA Janelia Fluor® 549, NHS ester

Tocris Bioscience | Catalog # 6149

Yellow photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
Tocris Bioscience

Key Product Details

Description

Yellow photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
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Product Description

Key information:

PA Janelia Fluor® 549, NHS ester is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.

Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.

Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed with PA Janelia Fluor® 646, NHS ester (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was PA Janelia Fluor® 549, SE.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Quantum Yield (φ) 0.88
Reactive Group NHS ester
Reactivity Primary amines

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Product Specifications for PA Janelia Fluor® 549, NHS ester

Molecular Weight

575.58

Formula

C32H25N5O6

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate

CAS Number

1811539-42-0

PubChem ID

126569714

InChI Key

ZJZWRNGNJDEPEQ-UHFFFAOYSA-N

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 5.76 10

Preparing Stock Solutions for PA Janelia Fluor® 549, NHS ester

The following data is based on the product molecular weight 575.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 17.37 mL 86.87 mL 173.74 mL
0.5 mM 3.47 mL 17.37 mL 34.75 mL
1 mM 1.74 mL 8.69 mL 17.37 mL
5 mM 0.35 mL 1.74 mL 3.47 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 3 publications citing the usage of this product.

Product Documents for PA Janelia Fluor® 549, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PA Janelia Fluor® 549, NHS ester

For research use only

Citations for PA Janelia Fluor® 549, NHS ester

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