PA Janelia Fluor® 646, NHS ester

Tocris Bioscience | Catalog # 6150

Red photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
Tocris Bioscience

Key Product Details

Description

Red photoactivatable dye (Reactive group: NHS ester). Application: super resolution microscopy (SRM), single molecule tracking and live cell imaging
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Product Description

Key information:

PA Janelia Fluor® 646, NHS ester is a photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.

Application: Suitable for single molecule tracking and super resolution microscopy in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.

Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed with PA Janelia Fluor® 549, NHS ester (Cat. No. 6149) to perform two-color sptPALM in live cells with activation at 405nm. Upon protein conjugation the efficiencies of photoconversion improve substantially. Low background staining. Excitation and emission maxima (λ) are 649 - 651 nm and 663 - 665 nm, respectively.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.

Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.

This product has been recently renamed. The previous name for this product was PA Janelia Fluor® 646, SE.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

Optical Data

Closest Laser line 640 nm
Reactive Group NHS ester

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Product Specifications for PA Janelia Fluor® 646, NHS ester

Molecular Weight

617.74

Formula

C34H31N5O5Si

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2'-diazo-5,5-dimethyl-2',3'-dihydro-5H-3'-oxospiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxylate

CAS Number

2093120-32-0

PubChem ID

127021042

InChI Key

XVIATAHKOZWKHH-UHFFFAOYSA-N

SMILES

O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5[Si](C)(C)C7=CC(N8CCC8)=CC=C47)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 12.35 20

Preparing Stock Solutions for PA Janelia Fluor® 646, NHS ester

The following data is based on the product molecular weight 617.74.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 8.09 mL 40.47 mL 80.94 mL
1 mM 1.62 mL 8.09 mL 16.19 mL
2 mM 0.81 mL 4.05 mL 8.09 mL
10 mM 0.16 mL 0.81 mL 1.62 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 3 publications citing the usage of this product.

Product Documents for PA Janelia Fluor® 646, NHS ester

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PA Janelia Fluor® 646, NHS ester

For research use only

Citations for PA Janelia Fluor® 646, NHS ester

Customer Reviews for PA Janelia Fluor® 646, NHS ester (2)

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Customer Images

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  • BG labelled PA JF646
    Name: Abigail Walker
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: HEK 293 cells
    Verified Customer | Posted 05/21/2024
    We conjugated PA JF656 with the SNAP label BG, and used it for single molecule imaging in HEK 293 cells (both live and fixed). The dye works very well, it is stable, bright, and activates and bleaches well (better than other photoactivatable dyes I have used). We have used it for imaging at 1-10 nM as we found some non-specific binding at concentrations higher than this, but potentially this could be removed with additional wash steps.
    PA Janelia Fluor® 646, NHS ester 6150
  • PA-JF646 for tracking single recombinant proteins
    Name: Anonymous
    Species: Bacteria
    Assay Type: In Vitro
    Verified Customer | Posted 02/26/2018
    This product was used to label recombinant proteins and image them with single molecule sensitivity. The degree of labeling achieved was 1.4 (average). The photoactivation efficiency of the dye is excellent and can be used to track a few molecules at a time, for long periods. The dye is highly photostable and can be used to track proteins for ~10-15 seconds under ideal tracking conditions. I attach here an image before and after photoactivation of the dye. Upon photoactivation, single dye molecules conjugated to the protein are visible.
    PA Janelia Fluor® 646, NHS ester 6150

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