PACT Antibody (1B9-1A7) - Azide and BSA Free
Novus Biologicals | Catalog # H00008575-M01
![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0013.jpg "Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B9-1A7
Format
Azide and BSA Free
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Product Specifications
Immunogen
PRKRA (AAH09470, 1 a.a. ~ 313 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK
Specificity
PRKRA - protein kinase, interferon-inducible double stranded RNA dependent activator
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PACT Antibody (1B9-1A7) - Azide and BSA Free
Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]
Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7. Analysis of PRKRA expression in human liver.![Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-Immunohistochemistry-Paraffin-H00008575-M01-img0009.jpg "Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01]")
Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01]
Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0012.jpg "Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]")
Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]
Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - PRKRA monoclonal antibody (M01), clone 1B9-1A7 Analysis of PRKRA expression in HeLa.![Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-Western-Blot-H00008575-M01-img0014.jpg "Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]")
Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01]
Western Blot: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA expression in transfected 293T cell line by PRKRA monoclonal antibody (M01), clone 1B9-1A7.Lane 1: PRKRA transfected lysate(34.4 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-Immunohistochemistry-Paraffin-H00008575-M01-img0007.jpg "Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01]")
Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01]
Immunohistochemistry-Paraffin: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of monoclonal antibody to PRKRA on formalin-fixed paraffin-embedded human squamous cell carcinoma. Antibody concentration 5 ug/ml.![Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-Immunoprecipitation-H00008575-M01-img0010.jpg "Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01]")
Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01]
Immunoprecipitation: PACT Antibody (1B9-1A7) [H00008575-M01] - Analysis of PRKRA transfected lysate using anti-PRKRA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRKRA MaxPab rabbit polyclonal antibody.![ELISA: PACT Antibody (1B9-1A7) [H00008575-M01]](https://beta-resources.rndsystems.com/images/products/PACT-Antibody-1B9-1A7-ELISA-H00008575-M01-img0015.jpg "ELISA: PACT Antibody (1B9-1A7) [H00008575-M01]")
ELISA: PACT Antibody (1B9-1A7) [H00008575-M01]
ELISA: PACT Antibody (1B9-1A7) [H00008575-M01] - Detection limit for recombinant GST tagged PRKRA is approximately 0.03ng/ml as a capture antibody.Applications for PACT Antibody (1B9-1A7) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PACT
Alternate Names
DYT16, HSD14, PKR-Associating Protein X, PRKRA, RAX
Entrez Gene IDs
8575 (Human)
Gene Symbol
PRKRA
OMIM
603424 (Human)
UniProt
Additional PACT Products
Product Documents for PACT Antibody (1B9-1A7) - Azide and BSA Free
Product Specific Notices for PACT Antibody (1B9-1A7) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PACT Antibody (1B9-1A7) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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