PACT Antibody (2W2E10)

Novus Biologicals | Catalog # NBP3-16701

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2W2E10 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 214-313 of human PACT (O75569). TWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PACT Antibody (2W2E10) (NBP3-16701) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PACT Antibody (2W2E10)

Western Blot: PACT Antibody (2W2E10) [NBP3-16701]

Western Blot: PACT Antibody (2W2E10) [NBP3-16701]

Western Blot: PACT Antibody (2W2E10) [NBP3-16701] - Western blot analysis of extracts of various cell lines, using PACTRabbit mAb (NBP3-16701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
PACT Antibody (2W2E10)

Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] -

Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded human brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
PACT Antibody (2W2E10)

Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] -

Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded mouse brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
PACT Antibody (2W2E10)

Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] -

Immunohistochemistry: PACT Antibody (2W2E10) [NBP3-16701] - Immunohistochemistry analysis of PACT in paraffin-embedded rat brain tissue using PACT Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for PACT Antibody (2W2E10)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PACT

PACT encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Alternate Names

DYT16, HSD14, PKR-Associating Protein X, PRKRA, RAX

Gene Symbol

PRKRA

Additional PACT Products

Product Documents for PACT Antibody (2W2E10)

Certificate of Analysis

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Product Specific Notices for PACT Antibody (2W2E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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