PADI4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-38499
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0005.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Loading...
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKKKSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PADI4 Antibody - BSA Free
![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0012.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow, liver, spleen and testis using Anti-PADI4 antibody NBP2-38499 (A) shows similar protein distribution across tissues to independent antibody NBP1-87353 (B).![Western Blot: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Western-Blot-NBP2-38499-img0006.jpg "Western Blot: PADI4 Antibody [NBP2-38499]")
Western Blot: PADI4 Antibody [NBP2-38499]
Western Blot: PADI4 Antibody [NBP2-38499] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0003.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow shows high expression.![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0004.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human kidney shows low expression as expected.![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0008.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human liver.![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0009.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human testis.![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0010.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human spleen.![Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]](https://resources.rndsystems.com/images/products/PADI4-Antibody-Immunohistochemistry-Paraffin-NBP2-38499-img0011.jpg "Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]")
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499]
Immunohistochemistry-Paraffin: PADI4 Antibody [NBP2-38499] - Staining of human bone marrow using Anti-PADI4 antibody NBP2-38499.Applications for PADI4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PADI4
Long Name
Peptidyl Arginine Deiminase type IV
Alternate Names
HL-60 PAD, PAD, PAD4, PDI4
Gene Symbol
PADI4
UniProt
Additional PADI4 Products
Product Documents for PADI4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PADI4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for PADI4 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review PADI4 Antibody - BSA Free and earn rewards!
Have you used PADI4 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...