PAF49 Antibody (635) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43633

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 635

Format

Azide and BSA Free
View all PAF49 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAF49. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse PAF49 Antibody (635) - Azide and BSA Free (NBP2-43633) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PAF49 Antibody (635) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: PAF49 Antibody (635) [NBP2-43633]

Immunocytochemistry/ Immunofluorescence: PAF49 Antibody (635) [NBP2-43633]

Immunocytochemistry/Immunofluorescence: PAF49 Antibody (635) [NBP2-43633] - Analysis of U2OS cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: CD3EAP protein stained by CD3EAP antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: PAF49 Antibody (635) [NBP2-43633]

Western Blot: PAF49 Antibody (635) [NBP2-43633]

Western Blot: PAF49 Antibody (635) [NBP2-43633] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5 % SDS-PAGE PAF49 antibody [635] dilution: 1:1000.
Western Blot: PAF49 Antibody (635) [NBP2-43633]

Western Blot: PAF49 Antibody (635) [NBP2-43633]

Western Blot: PAF49 Antibody (635) [NBP2-43633] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody [635]. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
PAF49 Antibody (635) - Azide and BSA Free

Western Blot: PAF49 Antibody (635) [NBP2-43633] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAF49 antibody (NBP2-43633) diluted at 1:1000.

Applications for PAF49 Antibody (635) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PAF49

PAF49/CAST is subunit of RNA polymerase I. PAF49/CAST has been shown to interact with activator upstream binding factor (UBF) and plays an important role in rDNA transcription.

Alternate Names

A34.5, antisense to ERCC 1, Antisense to ERCC-1 protein, ASE-1ASE1, CASTMGC118851, CD3 epsilon associated protein, CD3e antigen, epsilon polypeptide associated protein, CD3e molecule, epsilon associated protein, CD3E-associated protein, CD3-epsilon-associated protein, DNA-directed RNA polymerase I subunit RPA34, PAF49RNA polymerase I-associated factor PAF49

Gene Symbol

CD3EAP

Additional PAF49 Products

Product Documents for PAF49 Antibody (635) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PAF49 Antibody (635) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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