PAK6 [p Ser165] Antibody

Novus Biologicals | Catalog # NBP3-23266

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PAK6 Primary Antibodies »

Product Specifications

Immunogen

PAK6 (Ser-165) synthetic peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6. [UniProt# Q9NQU5]

Modification

p Ser165

Specificity

This antibody was cross-adsorbed to unphosphorylated PAK6 (Ser-165) peptide then affinity purified using phospho-PAK6 (Ser-165) peptide. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-PAK6 (Ser-165) peptide and also by treatment with lambda phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PAK6 [p Ser165] Antibody

PAK6 [p Ser165] Antibody

Western Blot: PAK6 [p Ser165] Antibody [NBP3-23266] -

Western Blot: PAK6 [p Ser165] Antibody [NBP3-23266] - Western blots of human PAK6 recombinant protein phosphorylated by ERK2. The blot was exposed to lambda phosphatase (lanes 2 & 6) then probed with anti-PAK6 (N-terminal) (lanes 1-4) or anti-PAK6 (Ser-165) phospho-specific (lanes 5-8). The antibodies were used in the presence of unrelated (lane 3) and PAK6 (N-terminal) (lane 4) peptide or PAK6 (Ser-165) (lane 7) and unrelated phospho-serine (lane 8) peptides, respectively.

Applications for PAK6 [p Ser165] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PAK6

PAK6, a novel AR-interacting protein, shares a high degree of sequence similarity with other p21-activated kinases (PAK). PAK6 co-translocates into the nucleus with AR in response to androgen. In addition, AR and ER alpha transcriptional activites were inhibited by PAK6 in transient transfections with episomal and integrated reporter genes. PAK, which is highly expressed in testis and prostate tissues, could contribute to the effects of tamoxifen in breast cancer and in other tissues.

Long Name

p21/Cdc42/Rac1-activated Kinase 6

Alternate Names

EC 2.7.11, p21 protein (Cdc42/Rac)-activated kinase 6, p21(CDKN1A)-activated kinase 6, p21-activated kinase 6, p21-activated protein kinase 6, PAK-5, PAK5EC 2.7.11.1, PAK-6, serine/threonine-protein kinase PAK 6

Gene Symbol

PAK6

Additional PAK6 Products

Product Documents for PAK6 [p Ser165] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PAK6 [p Ser165] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Customer Reviews for PAK6 [p Ser165] Antibody

There are currently no reviews for this product. Be the first to review PAK6 [p Ser165] Antibody and earn rewards!

Have you used PAK6 [p Ser165] Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies