Palladin Antibody (JE66-56)

Novus Biologicals | Catalog # NBP3-32709

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE66-56 expressed in HEK293
View all Palladin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Palladin aa 1-100 / 1,383. (Uniprot: Q8WX93)

Localization

Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, Z line.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

151 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Palladin Antibody (JE66-56)

Palladin Antibody (JE66-56)

Western Blot: Palladin Antibody (JE66-56) [NBP3-32709]

Western Blot: Palladin Antibody (JE66-56) [NBP3-32709] - Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (NBP3-32709) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15ug/Lane)
Lane 2: SiHa cell lysate (10ug/Lane)
Lane 3: A431 cell lysate (15 ug/Lane)
Lane 4: SW480 cell lysate (30 ug/Lane)
Lane 5: 293T cell lysate (20 ug/Lane)
Lane 6: HEK-293 cell lysate (20 ug/Lane)
Lane 7: MDA-MB-231 cell lysate (20 ug/Lane)
Lane 8: MDA-MB-468 cell lysate (20 ug/Lane)
Lane 9: MCF7 cell lysate (20 ug/Lane)

Predicted band size: 151 kDa
Observed band size: 110/151 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32709) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Palladin Antibody (JE66-56)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Palladin

Palladin is a cytoskeletal protein required for organization of normal actin cytoskeleton. Palladin plays roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. Palladin may function as a scaffolding molecule with the potential to impact both actin polymerization and the assembly of existing actin filaments into higher-order arrays. It binds to proteins that bind to either monomeric or filamentous actin. It localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Palladin's different isoforms may have functional differences. Palladin is also involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation.

Alternate Names

PALLD, PNCA1, SIH002

Gene Symbol

PALLD

Additional Palladin Products

Product Documents for Palladin Antibody (JE66-56)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Palladin Antibody (JE66-56)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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