PALS1/MPP5 Antibody (OTI2D3)

Novus Biologicals | Catalog # NBP2-01880

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2D3
View all PALS1/MPP5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MPP5(NP_071919) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

77.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PALS1/MPP5 Antibody (OTI2D3)

Western Blot: MPP5 Antibody (2D3) [NBP2-01880] Analysis of extracts (35ug) from 9 different cell lines by using anti-MPP5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: PALS1/MPP5 Antibody (OTI2D3) [NBP2-01880]

Flow Cytometry: PALS1/MPP5 Antibody (OTI2D3) [NBP2-01880]

Flow Cytometry: MPP5 Antibody (2D3) [NBP2-01880] - Analysis of Jurkat cells, using anti-MPP5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: PALS1/MPP5 Antibody (OTI2D3) [NBP2-01880]

Western Blot: PALS1/MPP5 Antibody (OTI2D3) [NBP2-01880]

Western Blot: MPP5 Antibody (2D3) [NBP2-01880] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPP5.
Flow Cytometry: PALS1/MPP5 Antibody (OTI2D3) [NBP2-01880]

Flow Cytometry: PALS1/MPP5 Antibody (OTI2D3) [NBP2-01880]

Flow Cytometry: MPP5 Antibody (2D3) [NBP2-01880] - Analysis of Hela cells, using anti-MPP5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for PALS1/MPP5 Antibody (OTI2D3)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.88 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PALS1/MPP5

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001 [PubMed 11311936]). MPP5 is a member of the p55-like MAGUK subfamily.[supplied by OMIM].

Long Name

Protein Associated with Lin Seven 1

Alternate Names

MPP5, Stardust

Entrez Gene IDs

64398 (Human)

Gene Symbol

MPP5

Additional PALS1/MPP5 Products

Product Documents for PALS1/MPP5 Antibody (OTI2D3)

Certificate of Analysis

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Product Specific Notices for PALS1/MPP5 Antibody (OTI2D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epithelial Cell Polarity

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