Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

Novus Biologicals | Catalog # NB820-59244

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Product Specifications

Applications

Western Blot (10 ug - 20 ug)

Application Notes

Western Blot (10 ug to 20 ug per lane), Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Specificity

Total Protein - Human Adult Normal Tissue: Pancreas

Type

Tissue

Life Stage

Adult

Tissue

Digestive/Waste

Protein State

Native

Tissue Condition

Normal

Subcellular Fraction

Whole

Scientific Data Images for Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244]

Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244]

Western Blot: Human Pancreas Whole Tissue Lysate (Adult Whole Normal) [NB820-59244] - man Pancreas Whole Tissue Lysate (Adult Normal) [NB820-59244] - Image from verified customer review.

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Pancreas

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Additional Pancreas Products

Product Documents for Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

Customer Reviews for Human Pancreas Whole Tissue Lysate (Adult Whole Normal) (1)

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Customer Images

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  • Name: Ming Lu
    Application: Western Blot
    Sample Tested: Human normal pancreas lysate
    Species: Human
    Verified Customer | Posted 09/15/2014
    Pancreas Normal Tissue
    Human Pancreas Whole Tissue Lysate (Adult Whole Normal) NB820-59244

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FAQs for Human Pancreas Whole Tissue Lysate (Adult Whole Normal)

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    • Q: I am interested in Whole Normal Pancreas Tissue Lysate (Adult Normal) (NB820-59244). Would you please let me know how much protein you recommend to load on the gel?
      A: This lysate should be prepared and ready to load in Western Blot. We recommend loading 20-40 ug of protein into your well to use as a positive control.

    • Q: Would you provide these information listed below? 1. The procedure of this lysate preparation. 2. The recipes of the buffer. ( component and concentration) In the data sheet, it only list the component: HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
      A: The lysate was prepared using Biochain's total protein extraction kit (cat # K3011010). The buffer composition is listed on the datasheet however I am unable to disclose the amount of each component as that information is proprietary.
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