PARP/Apoptosis Chemiluminescent Assay Kit

For the measurment of PARP-1 activity in cell extracts
Catalog # Availability Size / Price Qty
4685-096-K

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Product Details
Citations (1)
FAQs
Reviews (1)

PARP/Apoptosis Chemiluminescent Assay Kit Summary

Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.
 

Key Benefits

• Chemiluminescent readout
• 96 well format
• Highly sensitive – detects 0.1 mU PARP ~500 cells
• Dynamic range between 0.1 to 10 mU PARP
• Requires 10-100 ng extract for detection
• Assay Time ~3 hrs

Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?

This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.  During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

Product Specifications

  • Measure PARP-1 activity in cell extracts before and after apoptosis.
  • For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 10X Activated DNA
• PARP-HSA, 10 mUnits/µl
• 20 mM NAD
• Anti-PAR monoclonal Antibody
• Goat anti-mouse IgG-HRP
• 10 mM Etoposide
• 20X I-PAR Assay Buffer
• PARP PeroxyGlow A
• PARP PeroxyGlow™ B
• 5X Antibody Diluent
• Histone-Coated White Strip Well Plate, I-PAR

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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Citation for PARP/Apoptosis Chemiluminescent Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MUC1-C INTEGRATES CHROMATIN REMODELING AND PARP1 ACTIVITY IN THE DNA DAMAGE RESPONSE OF TRIPLE-NEGATIVE BREAST CANCER CELLS
    Authors: M Yamamoto, C Jin, T Hata, Y Yasumizu, Y Zhang, D Hong, T Maeda, M Miyo, M Hiraki, Y Suzuki, K Hinohara, H Rajabi, D Kufe
    Cancer Res., 2019;0(0):.  2019

FAQs

  1. Is it possible to purchase a smaller version of the PARP/Apoptosis Chemiluminescent Assay Kit?

    • R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

  2. Is it possible to test partial plates when using the PARP/Apoptosis Chemiluminescent Assay Kit?

    • The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

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PARP/Apoptosis Chemiluminescent Assay Kit
By Anonymous on 10/14/2022

The PARP/Apoptosis Chemiluminescent Assay was used to assess signs of apoptotic induction by Temozolomide in Temozolomide-Resistant versus parental glioma lines. Results were consistent.