PARP/Apoptosis Chemiluminescent Assay Kit

R&D Systems | Catalog # 4685-096-K

For the measurment of PARP-1 activity in cell extracts
R&D Systems
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Key Product Details

Features

Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

  • Chemiluminescent readout
  • 96 well format
  • Highly sensitive – detects 0.1 mU PARP ~500 cells
  • Dynamic range between 0.1 to 10 mU PARP
  • Requires 10-100 ng extract for detection
  • Assay Time ~3 hrs

Species

Multi-Species

Product Summary for PARP/Apoptosis Chemiluminescent Assay Kit

Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?
This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.  During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.
Product Specifications
  • Measure PARP-1 activity in cell extracts before and after apoptosis.
  • For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents for PARP/Apoptosis Chemiluminescent Assay Kit

  • 10X Activated DNA
  • PARP-HSA, 10 mUnits/µl
  • 20 mM NAD
  • Anti-PAR monoclonal Antibody
  • Goat anti-mouse IgG-HRP
  • 10 mM Etoposide
  • 20X I-PAR Assay Buffer
  • PARP PeroxyGlow A
  • PARP PeroxyGlow™ B
  • 5X Antibody Diluent
  • Histone-Coated White Strip Well Plate, I-PAR

Formulation, Preparation, and Storage

Shipping

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: PARP

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name

Poly [ADP-ribose] Polymerase

Alternate Names

ADPRT, PARP1, PPOL

Additional PARP Products

Product Documents for PARP/Apoptosis Chemiluminescent Assay Kit

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Citations for PARP/Apoptosis Chemiluminescent Assay Kit

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  • PARP/Apoptosis Chemiluminescent Assay Kit
    Name: Anonymous
    Verified Customer | Posted 10/14/2022
    The PARP/Apoptosis Chemiluminescent Assay was used to assess signs of apoptotic induction by Temozolomide in Temozolomide-Resistant versus parental glioma lines. Results were consistent.

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FAQs for PARP/Apoptosis Chemiluminescent Assay Kit

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  • Q: Is it possible to purchase a smaller version of the PARP/Apoptosis Chemiluminescent Assay Kit?

    A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

  • Q: Is it possible to test partial plates when using the PARP/Apoptosis Chemiluminescent Assay Kit?

    A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

  • Q: Is it possible to purchase a smaller version of the PARP/Apoptosis Chemiluminescent Assay Kit?

    A: R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.

  • Q: Is it possible to test partial plates when using the PARP/Apoptosis Chemiluminescent Assay Kit?

    A: The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

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