PC-12 Whole Cell Lysate

Name: PC-12 Whole Cell Lysate
Brand: Novus Biologicals
SKU: NBP2-13170
Price: 219 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-13170

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Product Documents

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Rat

Applications

Immunoprecipitation, Western Blot

Product Specifications

Application Notes

This product is not supplied in loading buffer. If you intend to us this product in Western Blot, we recommend 1 x sample buffer with 5% 2-Mercaptoethanol (BME) or other reducing agent.

Specificity

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (non-denatured)

Type

Cell

Tissue

Nervous

Protein State

Native

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

Standard RIPA buffer including protease and phosphotase inhibitors and 0.1% SDS

Preservative

No Preservative

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: PC-12

PC12 is a cell line derived from a pheochromocytoma of a male rat adrenal gland. PC12 cells are small, irregularly shaped cells that form as floating clusters with a few scattered attached cells in culture. PC12 cells are also suitable as a transfection host. With the addition of nerve growth factor to PC12 cells, they will stop dividing and terminally differentiate, making PC12 a useful model for neuronal differentiation.

Additional PC-12 Products

Product Documents for PC-12 Whole Cell Lysate

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Certificate of Analysis

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Product Specific Notices for PC-12 Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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