PCMT1 Antibody (7G6) - BSA Free

Novus Biologicals | Catalog # NBP3-43014

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7G6

Format

BSA Free
View all PCMT1 Primary Antibodies »

Product Specifications

Immunogen

protein-L-isoaspartate(D-aspartate) O-methyltransferase - (Uniprot# P22061)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCMT1 Antibody (7G6) - BSA Free

PCMT1 Antibody (7G6)

Western Blot: PCMT1 Antibody (7G6) [NBP3-43014] -

Western Blot: PCMT1 Antibody (7G6) [NBP3-43014] - HeLa cells were subjected to SDS PAGE followed by western blot with (PCMT1 Antibody) at dilution of 1:1000

Applications for PCMT1 Antibody (7G6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PCMT1

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]

Alternate Names

EC 2.1.1, EC 2.1.1.77, L-isoaspartyl protein carboxyl methyltransferase, L-isoaspartyl/D-aspartyl methyltransferase, PIMT, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, protein-L-isoaspartate (D-aspartate) O-methyltransferase, protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene Symbol

PCMT1

Additional PCMT1 Products

Product Documents for PCMT1 Antibody (7G6) - BSA Free

Certificate of Analysis

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Product Specific Notices for PCMT1 Antibody (7G6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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