Human PCNA ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-18219

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and cell culture supernates (100 ul)

Sensitivity

0.055 ng/ml

Assay Range

0.39-25 ng/ml
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human PCNA in the sample, this Human PCNA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human PCNA concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Precision

Intra-Assay Precision (Precision within an assay) %CV 10

Inter-Assay Precision (Precision between assays) %CV 15

Recovery for Human PCNA ELISA Kit (Colorimetric)

Recovery

87-99%

Scientific Data Images for Human PCNA ELISA Kit (Colorimetric)

ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219]

ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219]

ELISA: Human PCNA ELISA Kit (Colorimetric) [NBP3-18219] - Standard Curve Reference

Kit Contents for Human PCNA ELISA Kit (Colorimetric)

  • Balance Solution
  • Enzyme Conjugate
  • Microtiter Plate
  • Standards
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for Human PCNA ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human PCNA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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