PCTAIRE1 Antibody (JE64-55)

Novus Biologicals | Catalog # NBP3-32727

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE64-55 expressed in HEK293
View all PCTAIRE1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human PCTAIRE1 aa 1-150 / 496. (Uniprot: Q00536)

Localization

Cytoplasm, Cytoplasmic vesicle, secretory vesicle, Cell membrane, Synapse, synaptosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PCTAIRE1 Antibody (JE64-55)

PCTAIRE1 Antibody (JE64-55)

Western Blot: PCTAIRE1 Antibody (JE64-55) [NBP3-32727]

Western Blot: PCTAIRE1 Antibody (JE64-55) [NBP3-32727] - Western blot analysis of PCTAIRE1 on different lysates with Rabbit anti-PCTAIRE1 antibody (NBP3-32727) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat pancreas tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 56 kDa
Observed band size: 56/63 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32727) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for PCTAIRE1 Antibody (JE64-55)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCTAIRE1

PCTAIRE1 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. There are three alternatively spliced transcript variants described for this gene.

Long Name

Cyclin-dependent kinase 16

Alternate Names

CDK16, PCTK1

Gene Symbol

CDK16

Additional PCTAIRE1 Products

Product Documents for PCTAIRE1 Antibody (JE64-55)

Certificate of Analysis

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Product Specific Notices for PCTAIRE1 Antibody (JE64-55)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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