PD 98059

Tocris Bioscience | Catalog # 1213

MEK inhibitor
Tocris Bioscience

Key Product Details

Description

MEK inhibitor
View all MEK Inhibitors »

Product Description

PD 98059 is an inhibitor of mitogen-activated protein kinase kinase (MKK / MEK). Acts by binding to the inactivated form of MEK, thereby preventing its phosphorylation by cRAF or MEK kinase (IC50 = 2-7 μM). Inhibits cell growth and proliferation in acute myelogenous leukemia (AML) cell lines; causes G1 arrest by blocking p53-dependent p21 induction. Enhances embryonic stem cell self-renewal.

Product Specifications for PD 98059

Molecular Weight

267.28

Formula

C16H13NO3

Storage

Store at RT

Purity

≥98% (HPLC)

Chemical Name

2-(2-Amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

CAS Number

167869-21-8

PubChem ID

9794588

InChI Key

PKDHDJBNEKXCBI-UHFFFAOYSA-N

SMILES

COC1=C(N)C(=CC=C1)C1=CC(=O)C2=CC=CC=C2O1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 6.68 25
Ethanol 1.34 5

Preparing Stock Solutions for PD 98059

The following data is based on the product molecular weight 267.28.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 14.97 mL 74.83 mL 149.66 mL
1.25 mM 2.99 mL 14.97 mL 29.93 mL
2.5 mM 1.50 mL 7.48 mL 14.97 mL
12.5 mM 0.30 mL 1.50 mL 2.99 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 568 publications citing the usage of this product.

Product Documents for PD 98059

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PD 98059

For research use only

Customer Reviews for PD 98059 (5)

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  • PD 98059 treatment
    Name: Xiaoyu Zhang
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: neuron cells
    Verified Customer | Posted 02/07/2024
    Cultured mouse neuron treated with PD 98059 (10uM) for 15 mins. MAP staining showed after treatment.
    PD 98059 1213
  • PD 98059 abrogated the LPA induced phosphorylation of AMPK.
    Name: lisha joshi
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: BV2 microglia cells
    Verified Customer | Posted 06/18/2019
    We investigate the effect of LPA on microglia metabolic states. Trying to unravel possible pathways that maybe involved in this process, we used inhibitors of MAPKs pathway. Inhibition of ERK (PD 98059; 10µM) decreased the LPA induced activation of pAMPK (among other proteins).
    PD 98059 1213
  • The role of ERK pathway in LPA induced pro-inflammatory phenotype in microglia cells
    Name: Joanna Plastira
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Primary murine microglia cells
    Verified Customer | Posted 06/08/2019
    The role of MAPK pathways (JNK, p38 and ERK) was studied in the LPA induced pro-inflammatory phenotype in microglia. In this case cells were incubated for the indicated time points with LPA (1µM) in the presence or absence of PD98059 (10µM) or PD184352 (10µM). A series of assays were performed. Inhibition of ERK pathway didn't seem to play a role in the LPA induced phenotype (here presented: pp65).
    PD 98059 1213
  • PD 98059 inhibits 15d-PGJ2 induced pp42/44 expression
    Name: Chintan Koyani
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: HL-1 cardiomyocytes
    Verified Customer | Posted 10/13/2018
    HL-1 cells were incubated with p38 MAPK inhibitor (10 µM SB203580, 25 µM PD169316) or p42/44 MAPK kinase inhibitor (25 µM PD98059) for 30 min prior to 15dPGJ2 treatment (15 µM, 1 h). Immunoreactive bands for pp38 and pp42/44 MAPK expressions were followed by Western blot. PD98059 completely inhibited 15d-PGJ2 induced p42/44 phosphorylation (shown in Image).
    PD 98059 1213
  • Inhibition of MEK in ovarian theca cells by PD98059
    Name: Zhenmin Lei
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Ovarian theca cells
    Verified Customer | Posted 01/04/2018
    Used to treat mouse ovarian theca cells to inhibit MEK and detected by Western blot.

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