PDE4B Antibody (OTI1D12)

Novus Biologicals | Catalog # NBP2-01171

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D12
View all PDE4B Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDE4B(NP_002591) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

83.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDE4B Antibody (OTI1D12)

Western Blot: PDE4B Antibody (1D12) [NBP2-01171] Analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunocytochemistry/ Immunofluorescence: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunocytochemistry/Immunofluorescence: PDE4B Antibody (1D12) [NBP2-01171] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4B mouse monoclonal antibody.
Flow Cytometry: PDE4B Antibody (OTI1D12) [NBP2-01171]

Flow Cytometry: PDE4B Antibody (OTI1D12) [NBP2-01171]

Flow Cytometry: PDE4B Antibody (1D12) [NBP2-01171] - Analysis of Jurkat cells, using anti-PDE4B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: PDE4B Antibody (OTI1D12) [NBP2-01171]

Western Blot: PDE4B Antibody (OTI1D12) [NBP2-01171]

Western Blot: PDE4B Antibody (1D12) [NBP2-01171] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4B mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (OTI1D12) [NBP2-01171]

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human pancreas tissue using anti-PDE4B mouse monoclonal antibody.
Flow Cytometry: PDE4B Antibody (OTI1D12) [NBP2-01171]

Flow Cytometry: PDE4B Antibody (OTI1D12) [NBP2-01171]

Flow Cytometry: PDE4B Antibody (1D12) [NBP2-01171] - Analysis of Hela cells, using anti-PDE4B antibody, (Red), compared to a nonspecific negative control antibody (Blue).
PDE4B Antibody (OTI1D12)

Western Blot: PDE4B Antibody (OTI1D12) [NBP2-01171] -

PDE4B expression is up-regulated in the abdominal aortas from AngII-infused ApoE−/− mice. ApoE−/− mice were infused with AngII (1000 ng/kg/min) or saline solution for 28 days. (A) The PDE4B expression in abdominal aortas from these animals was assessed by Western blot. The levels of beta -actin are shown as a loading control. The boxplot on the right shows the quantification of the PDE4B protein levels. The box extends from the 25th to the 75th percentile, and the median is indicated by a horizontal line. The whiskers represent the maximum and minimum values (Saline, n = 4; Ang II n = 6); * p < 0.01 vs. saline. (B) Representative PDE4B immunostaining in these samples. The arrowheads indicate the PD4B-positive cells in aneurysmal tissues. Bars: 20 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33809405), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PDE4B Antibody (OTI1D12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.62 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDE4B

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4B

Alternate Names

DPDE4, PDE32

Entrez Gene IDs

5142 (Human)

Gene Symbol

PDE4B

Additional PDE4B Products

Product Documents for PDE4B Antibody (OTI1D12)

Certificate of Analysis

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Product Specific Notices for PDE4B Antibody (OTI1D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDE4B Antibody (OTI1D12)

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Protocols

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