PDK2 Antibody (2G1) - Azide and BSA Free

Novus Biologicals | Catalog # H00005164-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G1

Format

Azide and BSA Free
View all PDK2 Primary Antibodies »

Product Specifications

Immunogen

PDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM

Specificity

PDK2 - pyruvate dehydrogenase kinase, isoenzyme 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDK2 Antibody (2G1) - Azide and BSA Free

Western Blot: PDK2 Antibody (2G1) [H00005164-M01]

Western Blot: PDK2 Antibody (2G1) [H00005164-M01]

Western Blot: PDK2 Antibody (2G1) [H00005164-M01] - Analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody (M01), clone 2G1.Lane 1: PDK2 transfected lysate(46.2 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: PDK2 Antibody (2G1) [H00005164-M01]

Immunohistochemistry-Paraffin: PDK2 Antibody (2G1) [H00005164-M01]

Immunohistochemistry-Paraffin: PDK2 Antibody (2G1) [H00005164-M01] - Analysis of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 0.8 ug/ml.
Western Blot: PDK2 Antibody (2G1) [H00005164-M01]

Western Blot: PDK2 Antibody (2G1) [H00005164-M01]

Western Blot: PDK2 Antibody (2G1) [H00005164-M01] - Analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi ( Cat # H00005164-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody (M01) clone 2G1 (Cat # H00005164-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: PDK2 Antibody (2G1) [H00005164-M01]

Western Blot: PDK2 Antibody (2G1) [H00005164-M01]

Western Blot: PDK2 Antibody (2G1) [H00005164-M01] - PDK2 monoclonal antibody (M01), clone 2G1 Analysis of PDK2 expression in U-2 OS.
Sandwich ELISA: PDK2 Antibody (2G1) [H00005164-M01] - Detection limit for recombinant GST tagged PDK2 is approximately 0.3ng/ml as a capture antibody.

Applications for PDK2 Antibody (2G1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

0.8ug/ml

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDK2

PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Long Name

[Pyruvate dehydrogenase

Alternate Names

PDH kinase 2, PDHK2, PDKII

Entrez Gene IDs

5164 (Human)

Gene Symbol

PDK2

OMIM

602525 (Human)

UniProt

AAH05811 (Human)

Additional PDK2 Products

Product Documents for PDK2 Antibody (2G1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PDK2 Antibody (2G1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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