PDK2 Antibody (5F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00005164-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5F8

Format

Azide and BSA Free
View all PDK2 Primary Antibodies »

Product Specifications

Immunogen

PDK2 (AAH05811, 187 a.a. ~ 276 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HIGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVM

Specificity

PDK2 (5F8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PDK2 Antibody (5F8) - Azide and BSA Free

Western Blot: PDK2 Antibody (5F8) [H00005164-M02]

Western Blot: PDK2 Antibody (5F8) [H00005164-M02]

Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8. Analysis of PDK2 expression in A-431.
Immunohistochemistry-Paraffin: PDK2 Antibody (5F8) [H00005164-M02]

Immunohistochemistry-Paraffin: PDK2 Antibody (5F8) [H00005164-M02]

Immunohistochemistry-Paraffin: PDK2 Antibody (5F8) [H00005164-M02] - Analysis of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 2 ug/ml.
Western Blot: PDK2 Antibody (5F8) [H00005164-M02]

Western Blot: PDK2 Antibody (5F8) [H00005164-M02]

Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8 Analysis of PDK2 expression in HeLa.
Western Blot: PDK2 Antibody (5F8) [H00005164-M02]

Western Blot: PDK2 Antibody (5F8) [H00005164-M02]

Western Blot: PDK2 Antibody (5F8) [H00005164-M02] - PDK2 monoclonal antibody (M02), clone 5F8. Analysis of PDK2 expression in 293.
ELISA: PDK2 Antibody (5F8) [H00005164-M02]

ELISA: PDK2 Antibody (5F8) [H00005164-M02]

ELISA: PDK2 Antibody (5F8) [H00005164-M02] - Detection limit for recombinant GST tagged PDK2 is approximately 0.03ng/ml as a capture antibody.

Applications for PDK2 Antibody (5F8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1:100-1:2000
Application Notes
WB usage on cell lysate and recombinant protein reported in scientific literature (PMID: 22123926).

Reviewed Applications

Read 1 review rated 3 using H00005164-M02 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDK2

PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Long Name

[Pyruvate dehydrogenase

Alternate Names

PDH kinase 2, PDHK2, PDKII

Entrez Gene IDs

5164 (Human)

Gene Symbol

PDK2

OMIM

602525 (Human)

UniProt

AAH05811 (Human)

Additional PDK2 Products

Product Documents for PDK2 Antibody (5F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PDK2 Antibody (5F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDK2 Antibody (5F8) - Azide and BSA Free

Customer Reviews for PDK2 Antibody (5F8) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 08/22/2015

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