Rat PDK4 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81233

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 ng/mL

Assay Range

0.16 - 10 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat PDK4 in samples. No significant cross-reactivity or interference between Rat PDK4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.06%

Inter-Assay Precision (Precision between assays) CV% 5.08%

Recovery for Rat PDK4 ELISA Kit (Colorimetric)

Recovery

86-103%

Linearity

Rat PDK4 ELISA Kit (Colorimetric)

Scientific Data Images for Rat PDK4 ELISA Kit (Colorimetric)

Rat PDK4 ELISA Kit (Colorimetric)

ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] -

ELISA: Rat PDK4 ELISA Kit (Colorimetric) [NBP2-81233] - Standard Curve Reference

Kit Contents for Rat PDK4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PDK4

Pyruvate dehydrogenase kinase isoform 4 (PDK4) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

PDK4 antibodies are useful tools for glucose metabolism research and mitochondiral metabolism studies.

Alternate Names

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial, EC 2.7.11, EC 2.7.11.2, FLJ40832, Pyruvate dehydrogenase kinase isoform 4, pyruvate dehydrogenase kinase, isoenzyme 4, pyruvate dehydrogenase kinase, isozyme 4, pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Gene Symbol

PDK4

Additional PDK4 Products

Product Documents for Rat PDK4 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat PDK4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Rat PDK4 ELISA Kit (Colorimetric)

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  • Q: Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.

    A: NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.

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