Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PDLIM1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PDLIM1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PDLIM1 Antibody - BSA Free

Western Blot: PDLIM1 Antibody [NBP2-98546]

Western Blot: PDLIM1 Antibody [NBP2-98546]

Western Blot: PDLIM1 Antibody [NBP2-98546] - Anti-PDLIM1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lane D: Caco-2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa
Immunoprecipitation: PDLIM1 Antibody [NBP2-98546]

Immunoprecipitation: PDLIM1 Antibody [NBP2-98546]

Immunoprecipitation: PDLIM1 Antibody [NBP2-98546] - PDLIM1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 4 ul anti-PDLIM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PDLIM1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 36 kDa

Applications for PDLIM1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PDLIM1

CLP36 is a PDZ-domain and LIM-domain containing protein that associates with actin filaments and stress fibers via alpha-actinin. CLP36 has been show to be critical to stress fiber formation and focal adhesions. Alternative names for CLP36 include Elfin, C-terminal LIM domain protein 1, PDZ and LIM domain protein 1, CLIM1, and PDLIM1.

Alternate Names

CLIM1carboxyl terminal LIM domain protein 1, CLP-36, CLP36carboxy terminal LIM domain protein 1, C-terminal LIM domain protein 1, elfin, enigma, hCLIM1, LIM domain protein CLP-36, PDZ and LIM domain 1, PDZ and LIM domain 1 (elfin), PDZ and LIM domain protein 1

Gene Symbol

PDLIM1

Additional PDLIM1 Products

Product Documents for PDLIM1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PDLIM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PDLIM1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PDLIM1 Antibody - BSA Free and earn rewards!

Have you used PDLIM1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies