PDLIM7 Antibody (2D6) - Azide and BSA Free

Novus Biologicals | Catalog # H00009260-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D6

Format

Azide and BSA Free
View all PDLIM7 Primary Antibodies »

Product Specifications

Immunogen

PDLIM7 (AAH01093.1, 1 a.a. ~ 457 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGVPYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFSHV

Specificity

PDLIM7 - PDZ and LIM domain 7 (enigma)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for PDLIM7 Antibody (2D6) - Azide and BSA Free

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01]

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01]

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - PDLIM7 monoclonal antibody (M01), clone 2D6 Analysis of PDLIM7 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: PDLIM7 Antibody (2D6) [H00009260-M01]

Immunocytochemistry/ Immunofluorescence: PDLIM7 Antibody (2D6) [H00009260-M01]

Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of monoclonal antibody to PDLIM7 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01]

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01]

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 over-expressed 293 cell line, cotransfected with PDLIM7 Validated Chimera RNAi ( Cat # H00009260-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PDLIM7 monoclonal antibody (M01), clone 2D6 (Cat # H00009260-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01]

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01]

Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 monoclonal antibody (M01), clone 2D6.Lane 1: PDLIM7 transfected lysate(49.8 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: PDLIM7 Antibody (2D6) [H00009260-M01]

Immunoprecipitation: PDLIM7 Antibody (2D6) [H00009260-M01]

Immunoprecipitation: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 transfected lysate using anti-PDLIM7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PDLIM7 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: PDLIM7 Antibody (2D6) [H00009260-M01] - Detection limit for recombinant GST tagged PDLIM7 is approximately 0.3ng/ml as a capture antibody.

Applications for PDLIM7 Antibody (2D6) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PDLIM7

The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.

Alternate Names

1110003B01Rik, ENIGMAProtein enigma, LIM domain protein, LIM mineralization protein, LMP, LMP1, PDZ and LIM domain 7 (enigma), PDZ and LIM domain protein 7

Entrez Gene IDs

9260 (Human)

Gene Symbol

PDLIM7

OMIM

605903 (Human)

UniProt

AAH01093 (Human)

Additional PDLIM7 Products

Product Documents for PDLIM7 Antibody (2D6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PDLIM7 Antibody (2D6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PDLIM7 Antibody (2D6) - Azide and BSA Free

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Protocols

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FAQs

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