PEDFR/PNPLA2/ATGL Antibody (10Y4M10)

Novus Biologicals | Catalog # NBP3-16567

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10Y4M10 expressed in HEK293
View all PEDFR/PNPLA2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-394 of human ATGL / PNPLA2 (NP_065109.1). LPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEDFR/PNPLA2/ATGL Antibody (10Y4M10)

Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567]

Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567]

Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567] - Analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
PEDFR/PNPLA2/ATGL Antibody (10Y4M10)

Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567] -

Western Blot: PEDFR/PNPLA2/ATGL Antibody (10Y4M10) [NBP3-16567] - Western blot analysis of various lysates using PEDFR/PNPLA2/ATGL Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for PEDFR/PNPLA2/ATGL Antibody (10Y4M10)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PEDFR/PNPLA2

Patatin-like phospholipase domain-containing protein 2 (PNPLA2), also known as adipose triglyceride lipase (ATGL), is a lipolytic enzyme required for mobilization of fatty acids from triglyceride stores in adipose tissue. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. However, ATGL has now been shown to catalyze the initial step of triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Thus, ATGL and HSL coordinate to catabolize stored triglycerides in adipose tissue of mammals.

Defects in PNPLA2 can lead to neutral lipid storage disease with myopathy (NLSDM), a neutral lipid storage disorder with myopathy but without ichthyosis.

ATGL antibodies can serve as useful tools for adipocyte studies and research on triglyceride processing and fat storage.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Gene Symbol

PNPLA2

Additional PEDFR/PNPLA2 Products

Product Documents for PEDFR/PNPLA2/ATGL Antibody (10Y4M10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PEDFR/PNPLA2/ATGL Antibody (10Y4M10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes

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