PEDFR/PNPLA2/ATGL Antibody (1H6) - BSA Free

Novus Biologicals | Catalog # NBP3-26374

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1H6 expressed in HEK293

Format

BSA Free
View all PEDFR/PNPLA2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human PEDFR/PNPLA2/ATGL [UniProt Q96AD5]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEDFR/PNPLA2/ATGL Antibody (1H6) - BSA Free

PEDFR/PNPLA2/ATGL Antibody (1H6)

Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (1H6) [NBP3-26374] -

Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (1H6) [NBP3-26374] - Overlay Peak curve showing HepG2 cells stained with PEDFR/PNPLA2/ATGL Antibody (1H6) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for PEDFR/PNPLA2/ATGL Antibody (1H6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PEDFR/PNPLA2

Patatin-like phospholipase domain-containing protein 2 (PNPLA2), also known as adipose triglyceride lipase (ATGL), is a lipolytic enzyme required for mobilization of fatty acids from triglyceride stores in adipose tissue. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. However, ATGL has now been shown to catalyze the initial step of triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Thus, ATGL and HSL coordinate to catabolize stored triglycerides in adipose tissue of mammals.

Defects in PNPLA2 can lead to neutral lipid storage disease with myopathy (NLSDM), a neutral lipid storage disorder with myopathy but without ichthyosis.

ATGL antibodies can serve as useful tools for adipocyte studies and research on triglyceride processing and fat storage.

Long Name

Pigment Epithelium-derived Factor Receptor/Patatin-like Phospholipase Domain-containing Protein 2

Alternate Names

ATGL, Desnutrin, IPLA2-zeta, PEDF R, PNPLA2, TTS2

Gene Symbol

PNPLA2

Additional PEDFR/PNPLA2 Products

Product Documents for PEDFR/PNPLA2/ATGL Antibody (1H6) - BSA Free

Certificate of Analysis

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Product Specific Notices for PEDFR/PNPLA2/ATGL Antibody (1H6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes

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Protocols

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