Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-39288

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

13.5 pg/mL (example only; lot dependent)

Assay Range

31.25 - 2000 pg/mL (example only; lot dependent)
View all PAM ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images

Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric)

ELISA: Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric) [NBP3-39288]

ELISA: Human Peptidylglycine alpha-Amidating Monooxygenase/PAM ELISA Kit (Colorimetric) [NBP3-39288] - Standard Curve Reference

Kit Contents

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PAM

PAM encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq]

Long Name

Peptidylglycine Alpha-Amidating Monooxygenase

Alternate Names

PAL, PHM

Gene Symbol

PAM

Additional PAM Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

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